• Current and Future Transgenic Whole-Cell Biosensors for ...

     · Here, we review transgenic plant biosensors that have been used to detect macronutrients and micronutrients. As plant-based biosensors are limited by the requirement to introduce and optimize a transgene in every genotype of interest, we also review microbial biosensor cells that have been used to measure plant or soil nutrients by co ...

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    The element selenium (Se) is chemically similar to sulfur (S). For this reason plants and other organisms mistakenly take up and metabolize Se via S transporters and biochemical pathways. This can cause toxicity due to a combination of (1) oxidative stress caused directly by selenocompounds and (2) replacement of S by Se in proteins and other S compounds, which disrupts their function.

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  • gene Cloning & Transgenesis

    Keywords: Plants; Selenium; Se enrichment genes; Cloning; Transgenic Introduction Selenium (Se) was firstly found by Swedish scientist Jakob Berzelius in 1917. In the 1930s, it is generally accepted that Se is toxic or carcinogenic to human, while in 1957, Se was proved to be an important nutritional element of animal nutrition.

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  • Selenium Sponges | California Magazine

    Genetically modified organisms have earned few fans among the Prius-driving, organic food–buying set, who say that messing with genomes will be disastrous for ecosystems and human health. But a recent study by Berkeley scientist Norman Terry and colleagues might encourage green-minded types to consider the possible benefits of genetic engineering.

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  • Enhanced Selenium Tolerance and Accumulation in Transgenic ...

    The transgenic SL plants were analyzed with respect to Se incorpora-tion into proteins and Se tolerance and accumulation. RESULTS Transgenic Arabidopsis plants were obtained using the two gene constructs for cytosolic (Fig. 1A) and chloroplastic (Fig. 1B) expression, respectively. Ten transgenic lines per construct were identified using

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  • Selenium speciation in wild-type and genetically modified ...

    Some plants have the ability not only to grow in the presence of potential environmental contaminants, such as Se, but also to accumulate them (Se accumulators). As such, some of these plant species are excellent candidates for Se phytoremediation. One of the accumulation mechanisms for selenium …

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  • Selenium in Plants | SpringerLink

     · Abstract. Selenium (Se) and sulfur (S) are chemically similar. Most plants cannot discriminate between the two, with the exception of Se hyperaccumulator species, which preferentially accumulate Se over S. Genetic engineering of various genes from the S/Se assimilation pathway has successfully enhanced plant Se tolerance, accumulation, and volatilization, in both laboratory and field.

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  • Enhancing phytoremediation through the use of transgenics ...

     · The transgenic plants remained healthy; the wild-type plants grown in the selenium-contaminated soil had 50% less mass than in clean soil, while the GS transgenics had only a 20% reduction. Unfortunately, the US Department of Agriculture Animal and Plant Health Inspection Service (USDA-APHIS) allowed this field trial to be conducted for only 6 ...

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  • Transgenic mustard sucks up selenium : Nature News

     · Genetically modified Indian mustard plants have successfully cleaned up excessive selenium in a California field. This is the first field trial for a pollution-busting transgenic plant, and it ...

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  • Use of genetically engineered plants to decontaminate ...

    Selenium phytoremediation using genes from hyperaccumulator plants: ... The transgenic plants were able to grow well with root concentrations of Hg up to three-fold higher, depending on the form supplied, and were also able to volatilize elemental mercury to permanently remove the …

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  • Transgenic plants: Types, benefits, public concerns and ...

     · Transgenic plants have also been used for bioremediation of contaminated soils. Mercury, selenium and organic pollutants, like as polychlorinated biphenyls (PCBs), have been removed from soils by transgenic plants, containing genes for bacterial enzymes. 6. 3. Types. Transgenic plants have genes inserted into them, deriving from other species.

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  • Publication : USDA ARS

    All three transgenic lines accumulated and volatilized more selenium than the untransformed Indian mustard after 6 weeks of growth in the field. These results are significant because this is the first scientific report showing that plants genetically engineered for phytoremediation can perform successfully under field conditions nducting this ...

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  • Selenium in higher plants: understanding mechanisms for ...

    Selenium (Se) is an essential micronutrient for many organisms, including plants, animals and humans. As plants are the main source of dietary Se, plant Se metabolism is therefore important for Se nutrition of humans and other animals. However, the concentration of Se in plant foods varies between areas, and too much Se can lead to toxicity. As we discuss here, plant Se uptake and metabolism ...

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  • Phytoremediation of selenium using transgenic plants ...

    sulfur/selenium assimilation and volatilization, methylation of selenocysteine, and conversion of selenocysteine to elemental Se. Lab and field trials with different transgenic plants have yielded promising results, showing up to ninefold higher levels of selenium accumulation and up to threefold faster volatilization rates. Addresses

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  • Biotech Plants for Bioremediation | ISAAA

    Transgenic Arabidopsis plants expressing a selenocysteine methyltransferase (SMTA) gene from the selenium hyperaccumulator Astralagus bisulcatus contain eight times more selenium in their biomass when grown on selenite compared to non-transgenic controls.

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  • Selenium Metabolism in Plants: Molecular Approaches

    to two-fold in transgenic plants compared to wild type. These information show that sulfate transporter is involved in selena te uptake (Terry et al., 2000). Translocation of the Selenium in the plant parts depend on the form of how it is supplied. Zayed et al (1998) showed that the shoot/root ra tio of the Se concentrations ranged from 1.4

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  • Enhanced selenium tolerance and accumulation in transgenic ...

     · Phytoremediation of selenium using transgenic plants. Pilon-Smits EA, LeDuc DL. Curr Opin Biotechnol, 20(2):207-212, 09 Mar 2009 Cited by: 37 articles | PMID: 19269806. Review. Utilization of selenocysteine as a source of selenium for selenophosphate biosynthesis. Lacourciere GM ...

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  • Selenium accumulation by plants « Botany One

     · Selenium is not required by plants, but is an essential mineral element for animal nutrition. In excess, it can be harmful to both plants and animals. Selenium concentrations in vegetation can vary widely, depending upon both selenium phytoavailability in the soil and plant genotype. ... and transgenic approaches to the phytoremediation of land ...

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  • Enhanced Selenium Tolerance and Accumulation in Transgenic ...

    The transgenic Arabidopsis SL plants showed enhanced leaf SL enzyme activity levels, up to 6-fold for chloroplastic SL lines and up to 2-fold for cytosolic expressors (Fig. 2). The endogenous SL activity found in wild-type Arabidopsis plants (Figs. 2 and 3 ) may be explained by NifS-like proteins.

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  • Transgenic plants shown to clean up contaminated soil | UC ...

    In the field trial, the APS plant line absorbed 4.3 times more selenium than the wild-type plants. The other two transgenic lines were engineered to produce more of the enzymes gamma-glutamyl cysteine synthetase (ECS) and glutathione synthetase (GS), both of which play key roles in …

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  • Enhanced Selenium Tolerance and Accumulation in …

    Enhanced Selenium Tolerance and Accumulation in Transgenic Arabidopsis Expressing a Mouse Selenocysteine Lyasel Marinus Pilon, Jennifer D. Owen, Gulnara F. Garifullina, Tatsuo Kurihara, Hisaaki Mihara, ... in plant Se and/or S metabolism has yet to be eluci-dated (Pilon-Smits et al., 2002).

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  • Phytoremediation of selenium using transgenic plants ...

     · Several transgenic approaches have been used successfully to further enhance plant selenium accumulation, tolerance, and volatilization: upregulation of genes involved in sulfur/selenium assimilation and volatilization, methylation of selenocysteine, and conversion of selenocysteine to elemental Se. Lab and field trials with different ...

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  • Frontiers | An Overview of Selenium Uptake, Metabolism ...

     · Box 2. Transgenic approach. Selenium metabolism inside the plants can be manipulated using transgenic approach for effective Se-phytoremediation or biofortification. Prime focus should be to: • Increase plant tolerance of high soil Se concentration. • Increase Se uptake and transport to shoot. • Increase Se accumulation in shoot tissues.

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  • Enhanced Selenium Tolerance and Accumulation in …

    The transgenic SL plants were analyzed with respect to Se incorpora-tion into proteins and Se tolerance and accumulation. RESULTS Transgenic Arabidopsis plants were obtained using the two gene constructs for cytosolic (Fig. 1A) and chloroplastic (Fig. 1B) expression, respectively. Ten transgenic lines per construct were identified using

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  • Production of Se-methylselenocysteine in transgenic plants ...

     · Conclusion. These results demonstrate the feasibility of developing transgenic plant-based production of Se-methylselenocysteine, as well as bioengineering selenite resistance in plants.Selenite resistance is the first step in engineering plants that are resistant to selenate, the predominant form of Se in the environment.

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  • Transgenic strategies for the nutritional enhancement of ...

    compared to transgenic strategies is its reliance on alleles already in the species genepool. In some cases, this can be overcome by crossing to distant relatives and thus introgressing traits into commercial cultivars. Such a strategy could be used to increase selenium levels in wheat, because modern bread wheats show little

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  • Phytoremediation of selenium using transgenic plants ...

    Several transgenic approaches have been used successfully to further enhance plant selenium accumulation, tolerance, and volatilization: upregulation of genes involved in sulfur/selenium assimilation and volatilization, methylation of selenocysteine, and conversion of selenocysteine to elemental Se.

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  • Transgenic Plants Remove More Selenium From Contaminated ...

     · Transgenic Plants Remove More Selenium From Contaminated Soil Than Wild-type Plants, New Field Tests Show Date: February 9, 2005 Source: University Of California, Berkeley

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  • Accumulation of an organic anticancer selenium compound in ...

    Tolerance to high selenium (Se) soils in Se-hyperaccumulating plant species is correlated with the ability to biosynthesise methylselenocysteine (MeSeCys), due to the activity of selenocysteine methyltransferase (SMT). In mammals, inclusion of MeSeCys in the diet reduces the incidence of certain cancers, so increasing the range of crop plants that can produce this compound is an attractive...

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  • Selenium content, influential factors within the plant and ...

    The paper collected relevant literatures on selenium and explored the function to plant, selenium content, influential factors and selenium specification and transformation. We believed that there should be more deep research on function of selenium to plant. Approaches of molecular, genetic engineering and isotope could be employed to breed selenium rich crops and possibilities in practice.

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  • Publication : USDA ARS

    Transgenic Arabidopsis expressing BoCOQ5-2 volatilized three times more Se than the vector only control plants when treated with selenite and exhibited significant tolerance to selenium. BoCOQ5-2 represents the first plant enzyme that is not known to be directly involved in sulfur/selenium metabolism, yet mediates selenium volatilization.

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  • Examples of Transgenic Plants | Biotechnology

    Additional work suggested transgenic plant accumulate 4-17 fold greater fresh shoot weight and accumulated 2-3 fold more arsenic per gram of tissue than wild type (non-transformed) or plants expressing y-ECS or Ars C alone. Thus, transgenic technology led to the development of high biomass, fast growing arsenic hyper-accumulation. 3.

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  • Transgenic Plants in Phytoremediation: Recent Advances …

    Transgenic Plants in Phytoremediation: Recent ... sphere bacteria can enhance plant uptake of mercury and selenium (39). However, the exact mechanisms of these plant-microbe interactions are largely unknown. It is pos-sible that the microbe-mediated enhanced uptake may be

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  • Enhanced Selenium Tolerance and Accumulation in Transgenic ...

     · Selenium (Se) toxicity is thought to be due to nonspecific incorporation of selenocysteine (Se-Cys) into proteins, replacing Cys. In an attempt to direct Se flow away from incorporation into proteins, a mouse ( Mus musculus ) Se-Cys lyase (SL) was expressed in the cytosol or chloroplasts of Arabidopsis. This enzyme specifically catalyzes the decomposition of Se-Cys into elemental Se and ...

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  • The fascinating facets of plant selenium accumulation ...

    This step mainly occurs in plastids and has been reported to be rate limiting for Se assimilation as Brassica juncea transgenic plants overexpressing the isoform ATPS1 and provided with selenate exhibited a higher capacity to accumulate organic Se compared with the wild type and were more tolerant to Se (Pilon‐Smits et al., 1999). Such ...

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  • Transgenic mustard sucks up selenium | Nature

     · The transgenic plants showed up to 80% of the growth expected in uncontaminated soil, whereas the wild-type plants had their growth halved by the selenium.

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